Phlebotomy Education

The Order of Draw and Why It Exists

Cross-contamination of additives produces results that look real and are entirely wrong.

11 May 2026 · 5 min read

The sequence

Blood cultures, then citrate, then serum, then heparin, then EDTA, then fluoride oxalate — protecting the most sensitive assays first.

What goes wrong

EDTA carryover falsely raises potassium and lowers calcium; heparin carryover distorts coagulation studies.

Habit over memory

Lay tubes out in order before you start. A physical routine beats recall under pressure.

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